Fig. 4

COL1 is found in the PFL of central guttae. (A) The left and middle images show COL1 staining (red, polyclonal antibody from Rockland) of paraffin sections of corneas with guttae localized to the posterior fibrillar layer (PFL) surrounding central guttae as well as to the corneal stroma. The right image shows peripheral guttae without PFL but some remnants of CEnCs (blue is nuclear counter staining). (B) Paraffin sections of corneas with guttae stained for COL1 (red, antibody from Abcam) revealed staining localized to the PFL surrounding central guttae. Low concentration of the monoclonal antibody resulted in a highly specific signal. (C) Image of a paraffin section as in B. The COL1 signal co-localized with polarization signals observed under polarized light (POL). Unfortunately, the middle part of the image contains a fold in the section. (D) Flat-mounted Descemet membrane (DM) stained for COL1 is depicted using differential interference contrast (DIC), autofluorescence, and polarized light (POL). Regions with COL1 staining exhibited corresponding polarization signals and a fibrous structure visible in the DIC image. See also suppl. Fig. S4.