Fig. 1

Volcano plot (A) and Venn results (B) of dysregulated miRNAs in U937 cells at 24 h and 48 h post-infection. In volcano plot, values exceeding + 2 (log2FC) or −log10(FDR) > 10 were truncated for visualization. The Venn diagram illustrates the number of miRNAs whose expression is significantly altered at 24 h and 48 h following infection with L. infantum. Overlapping regions include miRNAs that are commonly dysregulated at different time points, while non-overlapping regions indicate miRNAs uniquely affected in that condition.