Fig. 3
Immune cells and glial cells in the inflamed CNS react to the respective therapy. Quantification of immune cells within the CNS of NOD EAE mice on day 63 treated with vehicle, FTY720 and vehicle, or FTY720 and nIFN-β daily from day 30 on (n = 10–12 mice per group). (A) Absolute cell number within the CNS (B) Absolute number of infiltrating monocytes (left) and Ly6C positive monocytes (right) within the CNS. (C) Absolute number of infiltrating CD4 T cells and the amount of IFN-γ and IL-17-producing CD4 T cells in the CNS. (D) Representative FACS plot of infiltrating IFN-γ and IL-17-producing CD4 + T cells. (E) Nos2 production of sorted astrocytes displayed by fold change in mRNA expression. (F) Absolute number of microglia within the CNS. (G) Quantification of CD4 positive T cells within the lymph node and the corresponding number of IFN-γ and IL-17-producing T cells. All data are mean ± SEM and representative of two independent experiments. Outliers were identified using ROUT with Q = 1%. P-values of p < 0.05 were considered significant and determined by Brown-Forsythe and Welch ANOVA tests. Dunnett`s T3 multiple comparison test was used to compute individual variances for each comparison.
