Fig. 1

Assessment of Off-Target Binding and Impact of C21 on Wheel-Running Activity. (a) Off-target binding affinities of the C21 at a concentration of 100 nM for membranes expressing receptors, channels and transporters. The values and abbreviations are shown in Table 1. (b) Influence of C21 at 0.002 and 0.01 mg/g of food on wheel-running activity. Each bar represents the mean + S.E.M. of daily wheel-running activity over a 3-wk period (n = 9). No significant differences were observed in the wheel-running activity of mice administered C21 at different concentrations compared to those given the vehicle. (c) Effect of C21 at 0.002 and 0.01 mg/g of food on delayed activity index on wheel running. Each bar represents the mean + S.E.M. of delayed activity index over a 3-wk period (n = 9). (d) Effect of C21 at 0.002 and 0.01 mg/g of food on body weight. Each circle indicates body weight of each mouse, and each bar represents the mean + S.E.M. of body weight after wheel-running activity for 2 weeks (n = 9 each). (e) Effect of C21 on food intake on wheel running. Each circle indicates food intake of each mouse, and each bar represents the mean + S.E.M. of food intake over a 2-wk period (n = 9 each). (f) Concentration (nM) of C21 in the forebrain after wheel-running activity measurement for 3 weeks (0.002 mg/g food group: n = 4; 0.01 mg/g food group: n = 5). Each bar represents the mean of concentration of C21. Each circle indicates the C21 concentration of each mouse.