Fig. 5
Heat maps depicting the relative abundance of dihydrosphingomyelins (a, DHSM), sphingomyelins (b, SM), glycosphingolipids (c), ceramides (d, Cer), monohexosylceramides (e, MonHex) and bis(monoacylglycerol)phosphate (f, BMP) in liver tissue (HFD/HFD n = 5, HFD/HFD + TM n = 5, HFD/HFD + TM + TRF n = 5, HFD/LFD n = 5, HFD/LFD + TM n = 5, HFD/HFD + TM + TRF n = 5). Lipid concentrations are quantified as pmol per mg of liver tissue. Color intensity reflects lipid abundance, with dark blue indicating high, light blue intermediate, and white low concentrations of the respective lipid species. Statistical significance was assessed using two-way ANOVA (lipid effect: a: p < 0.0001, F value (F) = 161.3, Degree of Freedom (DF) = 9; b: p < 0.0001, F = 223.3, DF = 9; c: p < 0.0001, F = 314.9, DF = 3; d: p < 0.0001, F = 270.7, DF = 7; e: p < 0.0001, F = 564.9, DF = 4; f: p < 0.0001, F = 173.5, DF = 7; intervention effect: a: p < 0.0001, F = 47.64, DF = 5; b: p < 0.0001, F = 34.85, DF = 5; c: p < 0.0001, F = 6.165, DF = 5; d: F = 0.5430, DF = 5; e: p < 0.0001, F = 12.51, DF = 5; f: p < 0.0001, F = 18.78, DF = 5 and interaction effect: a: p < 0.0001, F = 7.079, DF = 45; b: p < 0.0001, F = 8.738, DF = 45; c: p = 0.0030, F = 1.921, DF = 35; d: F = 0.6392, DF = 15; e: p < 0.0001, F = 2.637, DF = 20; f: p < 0.0001, F = 14.39, DF = 35; followed by Sidak or Tukey’s multiple comparisons test (p values in main text). Data are presented as mean and statistical significance was set at p < 0.05. HFD: high-fat diet, LFD: low-fat diet, TM: treadmill, TRF: time-restricted feeding. The table below the figure displays the individual groups, respectively. Table is read from top to bottom, ‘ + ’ denotes implementation of a given diet or intervention and ‘−’ its absence.
