Fig. 4
From: Lipidomics of prolonged labor duration in African American birthing people
Lipid modules associated with dystocia and their correlation with clinical outcomes. The bubble plot illustrating the log2 fold changes in lipid species between the dystocia and comparison group for (A) PE lipid species in module M6 and (F) TG species in module M8. Each point is plotted against total carbon length. Different colors represent the fatty acyl chains here: orange for saturated fatty acids; green for mono-unsaturated fatty acids (N9); blue for omega-3 (N3) fatty acids and red for omega-6 (N6) fatty acids. The black stroke indicates fold changes that achieves the statistical significance (p < 0.05). (B) Boxplots shows the ratio of peal area (PK)/internal standards(STD) of long chain (LC) and very long chain (VLC) Cer/HexCer in serum samples from comparison (Con) and dystocia (Dys) group across fatty acyl chain ranging from 14–24. p < 0.05 indicates statistical significance between group means using t-test. Bubble plot of carbon chain length vs. double bond for LPC lipid species in modules M10 (C) and M13 (D), showing log2 fold changes (dystocia/comparison). The intensity of the color gradient indicates the magnitude of the log2 fold change, while the size of the bubbles represents statistical significance. Boxplots of individual subjects within the PI lipid class of module M14, showing the sum of internal standards-normalized peak areas for the Comparison and Dystocia groups. Desaturation levels and carbon length by lipid class, and boxplots of individual subjects in the Comparison and Dystocia groups for different sub-lipid classes within the PI lipid class of module M14, shown in panels (E). (G) Heatmap showing the correlation results between clinical outcomes and lipid modules in the participants WGCNA was conducted using lipidomics data derived from all participants in African-American women cohort (n = 172; Visit 1: n = 82; Visit 2: n = 76; 72 participants attended both visits.). * p < 0.05; ** p < 0.01; *** p < 0.001. Labor_duration_adj: labor duration adjusted for parity and onset labor type. Hb: Hemoglobin.
