Fig. 1
From: Targeting cancer expressed EGFR with a humanized monoclonal antibody
Binding of the humanized antibodies to EGFR. (a) Binding of the humanized antibodies at a 2 µg/ml to MDA-MB-468 cells. The bar represented by A1 was the chimera. Data represents the average of three different experiments. p values comparing A8, A10 and B8 to the chimera are < 0.001. (b) The best EGFR binders were tested on cells stable expressing EGFRvIII (F98npEGFRvIII), wild type EGFR (WI38) and amplified EGFR (MDA-MB-468) at 0.5 and 2 µg/ml. Cetuximab was used at a concentration of 2 µg/ml. The control is represented by the unstained cells. The figure is representative of three experiments.
