Abstract
Impaired amyloid-β (Aβ) clearance and chronic neuroinflammation are central drivers of Alzheimer’s disease (AD) progression, yet therapeutic strategies targeting these processes remain limited. Proprotein convertase subtilisin/kexin type 9 (PCSK9) negatively regulates low-density lipoprotein receptor family members, including low-density lipoprotein receptor -related protein 1 (LRP1), a key mediator of blood-brain barrier (BBB) Aβ efflux, and has emerging roles in inflammatory signaling. In this study, to define the therapeutic relevance of PCSK9 inhibition in AD-like pathology, we examined SBC-115,076 in AlCl₃-challenged zebrafish larvae and mechanistically validated its effects using complementary endothelial–microglial in vitro models. SBC-115,076 significantly improved locomotor behavior and sensorimotor responsiveness, reduced Aβ deposition and neuronal apoptosis, and normalized oxidative stress, cholinergic dysfunction, and neuroinflammatory markers in vivo. Mechanistically, SBC-115,076 downregulated endothelial PCSK9 and upregulated LRP1, thereby enhancing Aβ endocytosis, lysosomal trafficking, and selective brain-to-blood efflux across an in vitro BBB model. In parallel, SBC-115,076 suppressed Aβ-induced CD36/TLR4 signaling in microglia, attenuated M1-like activation, promoted M2-like polarization, and reduced pro-inflammatory cytokine release. Collectively, these findings demonstrate that small-molecule PCSK9 inhibition ameliorates AD-like pathology through coordinated enhancement of BBB-mediated Aβ clearance and suppression of microglial inflammatory amplification, highlighting PCSK9 as a multifaceted therapeutic target and supporting a BBB-oriented strategy for AD intervention.
Data availability
The original contributions presented in the study are included in the article and/or Supplementary Material. The raw data supporting the conclusions of this article will be made available by the corresponding author upon reasonable request.
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Acknowledgements
We thank Prof. Zhenyan Song’s group at Hunan University of Chinese Medicine for guidance and assistance with zebrafish behavioral experiments.
Funding
This work was supported by National Natural Science Foundation of China (No. 32571692), Fundamental Research Program of Shanxi Province (Grant No. 202303021222348, 202303021221212), STI2030-Major Projects (2021ZD 0201801), and National Key Research and Development Program of China (2023YFC3605400).
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J.M., J.W. and W.H.Z. conceived the study. J.M. and J.W. designed the methodology and performed the formal analysis. J.M. wrote the main manuscript text. W.H.Z. and J.H.G. reviewed and edited the manuscript. J.H.G. and W.H.Z. supervised the project. J.H.G., W.H.Z. and J.M. acquired funding. All authors reviewed the manuscript.
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Miao, J., Wang, J., Zhou, W. et al. Small-molecule PCSK9 inhibition enhances BBB amyloid-β clearance and suppresses microglial inflammation in Alzheimer’s disease models. Sci Rep (2026). https://doi.org/10.1038/s41598-026-46671-y
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DOI: https://doi.org/10.1038/s41598-026-46671-y