Figure 5
Histological and digital autoradiography (DAR) analysis of brain sections from control and neuroinflammatory model mice. Hematoxylin and eosin (H&E) staining showing (a) an overview of the cerebrum (B) and cerebellum (C), (b) a 40 × magnification of the cerebellar cortex (10 µm section thickness) and (c) a 40 × magnification of the subsequent adjacent section of the same region (4 µm section thickness). The cerebellar cortical layers are indicated, including blood vessel (V), the granule cell layer (G), molecular layer (M), and Purkinje cell layer (P; black arrows indicate Purkinje cells). No apparent histopathological differences were observed between the experimental groups. DAR analysis and co-registered H&E and DAR (d,e) revealed a stronger cerebellar signal in the neuroinflammatory group compared with controls. Scale bars: (a and e) 500 µm; (b,c) 50 µm.
