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Prognostic value of point-of-care testing for cardiac myosin-binding protein C in early risk assessment of acute myocardial infarction: a prospective cohort study
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  • Published: 06 April 2026

Prognostic value of point-of-care testing for cardiac myosin-binding protein C in early risk assessment of acute myocardial infarction: a prospective cohort study

  • Zengguang Chen1 na1,
  • Jing Huang2 na1,
  • Jing Wang3,
  • Yiyang Zhan4 &
  • …
  • Qianwei Sun5 

Scientific Reports , Article number:  (2026) Cite this article

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We are providing an unedited version of this manuscript to give early access to its findings. Before final publication, the manuscript will undergo further editing. Please note there may be errors present which affect the content, and all legal disclaimers apply.

Subjects

  • Biomarkers
  • Cardiology
  • Diseases
  • Medical research
  • Risk factors

Abstract

The prognostic value of myocardial myosin-binding protein C (cMyC) in acute myocardial infarction (AMI) remains insufficiently studied. To evaluate the association between admission cMyC levels and the risk of 30-day major adverse cardiovascular events (MACE) in patients with AMI. In this prospective, single-center study, patients with AMI admitted between March 2022 and July 2024 were included. cMyC and hs-cTnI were measured by point-of-care testing (POCT) at admission. The primary endpoint was 30-day MACE. Predictive performance was evaluated using ROC analysis and multivariable Cox regression. A total of 285 patients were included, with a 30-day MACE incidence of 27.4%. Admission cMyC was independently associated with 30-day MACE after adjustment for clinical covariates. Exploratory analyses suggested a graded association between cMyC levels and the risk of adverse outcomes. Admission cMyC measured by POCT was independently associated with 30-day MACE in patients with AMI. cMyC may serve as a rapid adjunctive biomarker for early risk stratification, although its incremental predictive value requires further validation.

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Data availability

The clinical data used in this study are available from the corresponding author upon reasonable request, subject to ethical and privacy constraints. The data are not publicly available due to confidentiality agreements with participants.

Abbreviations

cMyC:

Cardiac myosin-binding protein C

AMI:

Acute myocardial infarction

MACE:

Major adverse cardiovascular events

POCT:

Point-of-care testing

AHF:

Acute heart failure

CHD:

Coronary heart disease

MI:

Myocardial infarction

CABG:

Coronary artery bypass grafting

STEMI:

ST-segment elevation myocardial infarction

pPCI:

Primary percutaneous coronary intervention

TC:

Total cholesterol

LDL-C:

Low-density lipoprotein cholesterol

CK-MB:

Creatine kinase isoenzymes

NT-proBNP:

N-terminal pro-brain natriuretic peptide

RBC:

Red blood cell count

WBC:

White blood cell count

N:

Neutrophil count

HB:

Hemoglobin

PLT:

Platelet count

hs-CRP:

High-sensitivity C-reactive protein

TG:

Triglycerides

HDL-C:

High-density lipoprotein cholesterol

Lp(a):

Lipoprotein (a)

BUN:

Blood urea nitrogen

Cr:

Creatinine

eGFR:

Estimated glomerular filtration rate

LVEDD:

Left ventricular end-diastolic diameter

LVEF:

Left ventricular ejection fraction

hs-cTnI:

High-sensitivity cardiac troponin I

EPV:

Event-per-variable

SD:

Standard deviation

ANOVA:

Analysis of variance

ROC:

Receiver operating characteristic

AUC:

Area under the curve

CI:

Confidence interval

C-index:

Concordance index

LASSO:

Least absolute shrinkage and selection operator

HR:

Hazard ratio

DCA:

Decision curve analysis

IDI:

Integrated discrimination improvement

NRI:

Net reclassification improvement

RCS:

Restricted cubic spline

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Funding

This study was supported by Changzhou Sci&Tech Program (Grant No. CJ20241073).

Author information

Author notes

  1. Zengguang Chen and Jing Huang contributed equally as first authors.

Authors and Affiliations

  1. Department of Cardiology, The Third Affiliated Hospital of Nanjing Medical University, The Second People’s Hospital of Changzhou, Changzhou, Jiangsu, China

    Zengguang Chen

  2. Graduate Department, Nanjing Medical University, Nanjing, Jiangsu, China

    Jing Huang

  3. Health Management Teaching Department, Changzhou Hygiene Vocational Technology College, Changzhou, Jiangsu, China

    Jing Wang

  4. Department of Geriatrics, The First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing, 210029, Jiangsu, China

    Yiyang Zhan

  5. Department of Anesthesiology, The Affiliated Hospital of Xuzhou Medical University, Quanshan District, 99 Huaihai West Road, Xuzhou, 221000, Jiangsu, China

    Qianwei Sun

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Contributions

Zengguang Chen and Jing Huang contributed equally to the study and wrote the main manuscript text. Jing Wang and Qianwei Sun contributed to the study design and methodology. Yiyang Zhan analyzed the data and provided statistical support. Zengguang Chen and Jing Huang performed the experiments and collected the data. Qianwei Sun and Yiyang Zhan supervised the study. Qianwei Sun also revised the manuscript. All authors reviewed and approved the manuscript.

Corresponding author

Correspondence to Qianwei Sun.

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The authors declare no competing interests.

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Chen, Z., Huang, J., Wang, J. et al. Prognostic value of point-of-care testing for cardiac myosin-binding protein C in early risk assessment of acute myocardial infarction: a prospective cohort study. Sci Rep (2026). https://doi.org/10.1038/s41598-026-47454-1

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  • Received: 06 February 2026

  • Accepted: 31 March 2026

  • Published: 06 April 2026

  • DOI: https://doi.org/10.1038/s41598-026-47454-1

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Keywords

  • Acute myocardial infarction
  • Myocardial myosin-binding protein C
  • Point-of-care test
  • Major adverse cardiovascular events
  • Risk assessment
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