Fig. 2

CRISPR-Cas9 knockout of C16orf87 does not affect Panc-01 cell viability. (A) Whole-cell lysates of Panc-01^KO and Panc-01^WT cells were analyzed by western blot (WB), and proteins were detected with the antibodies against C16orf87 and actin. An asterisk indicates the migration of the C16orf87 protein. Mw; molecular weight marker. (B) MS-based proteomics analysis of Panc-01^KO and Panc-01^WT cell lysates (FDR ≤ 0.05, n = 3). Data points representing histones and proteins of interest are highlighted in red. (C) Panc-01^KO and Panc-01^WT cell viability was analyzed by FACS after AnnexinV-FITC (AnnV) and DRAQ7 (Dq7) staining. (D) Panc-01^KO and Panc-01^WT cell proliferation was analyzed by FACS after EdU-A647 incorporation into cells. (E) Quantification of the FACS analysis (P < 0.01 (**)).