Abstract
Cotton Fusarium wilt, caused by Fusarium oxysporum f. sp. vasinfectum (Atk.), severely impairs cotton yield and quality. This study developed a novel eco-friendly seed coating agent (DIF) to replace toxic conventional formulations, with Difenoconazole as the core active ingredient. Laboratory and field trials (2024, Anhui) evaluated DIF’s effects on seed germination, seedling growth, Fusarium wilt control and cotton fibre quality, with carboxin-thiram (CT) as the positive control and uncoated seeds as blank control (CK). The 1:100 (agent: seed) DIF was optimal, boosting seedling root length (13.96 cm) and biomass, and achieving a 98.67% field control efficiency against Fusarium wilt. DIF improved seed yield and lint yield vs. CK (P > 0.05) and had no adverse effects on key fibre quality parameters. This eco-friendly DIF has great potential for cotton Fusarium wilt management with economic and environmental benefits.
Similar content being viewed by others
Funding
This research was supported by the Science Research Project of Anhui Academy of Agricultural Science of Agricultural, and National Modern Agricultural Industry Technology System Construction Special Project (CARS-15-32).
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Competing interests
The authors declare no competing interests.
Additional information
Publisher’s note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.
About this article
Cite this article
Chen, C., Li, S., Kan, H. et al. Development and application of a novel eco-friendly seed coating agent (DIF) for controlling cotton Fusarium wilt and improving seedling growth and yield. Sci Rep (2026). https://doi.org/10.1038/s41598-026-51869-1
Received:
Accepted:
Published:
DOI: https://doi.org/10.1038/s41598-026-51869-1


