Fig. 4

Microglia and macrophages in all brain regions express analyzed markers. Brain sections of HPI and TBI chimeras were stained for IBA1 (green; microglia/macrophages), and the respective markers (red). Microglia (MG) and macrophages (MP) were discriminated by GFP expression (MG: IBA1⁺GFP⁻, MP: IBA1⁺GFP⁺). a Graph depicts SALL1, TMEM119 and P2RY12 expression of all IBA1⁺ cells in different brain regions and under HPI and TBI condition. b Images show single cells of microglia and macrophages that express SALL1, TMEM119 and P2RY12. DAPI (blue, nuclei), GFP (violet, infiltrated cells). c Graphs depict calculation of microglia and macrophages that express SALL1, TMEM119 and P2RY12 in different brain regions under HPI (upper graph) and TBI (lower graph) condition. HPI head protected irradiation, TBI total body irradiation, MG microglia, MP macrophages, cl contralateral, pt peritumoral, it intratumoral, na not applicable. ANOVA and Bonferroni correction (n = 3–6), p* < 0.05, p** < 0.01, p*** < 0.001, ns not significant. An overview of the images and the corresponding negative controls is provided in Figs. S1 and S2.