Fig. 5

Primary microglia and primary macrophages express markers in vitro. a Scheme of the isolation and culture procedure for primary microglia and bone marrow-derived macrophages (created with BioRender.com). Microglia were isolated from naïve mouse brains and cultured for three days followed by fixation and staining. In addition, bone marrow cells were isolated, and cultured for eight days with M-CSF for differentiation of macrophages. Macrophages were taken and plated. After two days of culture, macrophages were fixed and stained. b Microglia and macrophages were stained for IBA1 (green; microglia/macrophages) and the respective markers (red). DAPI (blue; nuclei). Scale bars 100 μm. MG microglia, MP macrophages. Representative pictures of two independent experiments are shown (3–4 wells/marker in total, 8 images/well). An overview of the images and the corresponding negative controls is provided in Fig. S3.