Fig. 6

Microglial cells (BV2) downregulate markers after stimulation with tumor-conditioned medium. BV2 cells were cultured for three days in standard medium (Control) or tumor-conditioned medium (TCM). a Cells were fixed and stained with Phalloidin (green, F-Actin) and the respective markers (red). DAPI (blue, nuclei). Scale bars 100 μm. Representative images of three independent experiments are depicted. b-e Mean intensity of fluorescence was measured for each marker. Graphs depict intensity calculation for SALL1 (b), TMEM119 (c), P2RY12 (d) and HEXB (e). C control, TCM tumor-conditioned medium. Student`s t-test was used (n = 6–11 wells/marker in total), p* < 0.05, p*** < 0.001, p**** < 0.0001, ns not significant. An overview of the images and the corresponding negative controls is provided in Fig. S4.