Fig. 2 | npj Precision Oncology

Fig. 2

From: Enzyme-mediated depletion of l-cyst(e)ine synergizes with thioredoxin reductase inhibition for suppression of pancreatic tumor growth

Fig. 2The alternative text for this image may have been generated using AI.

Effects of cyst(e)inase on cell cycle progression and stress signaling. a Quantification of cell cycle phase distribution 24 h after cyst(e)inase treatment (n = 3 independent experiments). b Cell cycle phase distribution 48 h after cyst(e)inase treatment for Panc1 cells. c, d Quantification of cell cycle phase distribution for Panc1 c, MIA-PaCa2 and BxPC3 cells d 48 h after treatment (n = 3 independent experiments). e Regulatory cell cycle proteins following 24 and 48 h cyst(e)inase treatment. f, g Effect of treatment on mTORC1 signaling via the eIF2α-ATF4-Sestrin2 pathway in Panc1, BxPC3 f and MIA-PaCa2 g cells. h Effect of treatment on AMPK signaling. For e−h, “+” represents 250 nM cyst(e)inase treatment except for e, where “+” for Panc1-48 h represents 100 nM treatment. All data represent mean ± s.e.m. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, ns, not significant; compared to untreated controls; two-way ANOVA with Bonferroni’s method for multiple-comparison test. Experiment in (e−h) were repeated twice with similar results. All other experiments were repeated three times

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