Fig. 6 | npj Precision Oncology

Fig. 6

From: Enzyme-mediated depletion of l-cyst(e)ine synergizes with thioredoxin reductase inhibition for suppression of pancreatic tumor growth

Fig. 6The alternative text for this image may have been generated using AI.

Cyst(e)inase synergistically inhibits growth of pancreatic cancer xenografts in combination with auranofin without toxicity. a Growth of xenografted BxPC3 pancreatic tumors in male nude mice treated with vehicle control (n = 8 mice), cyst(e)inase (Cys, n = 8 mice), auranofin (Aur, n = 8 mice), or cyst(e)inase and auranofin in combination (n = 7 mice), and waterfall plots indicating the percent difference from median tumor volume of vehicle-treated group at day 42. b Average body weight (top), liver toxicity as assessed by serum alanine aminotransferase (ALT) activity (middle), and renal function as assessed by serum urea concentration (bottom) in mice from a. c, d Representative immunohistochemical staining c of xenografts from a, and their quantification d, which was performed in ImageJ software. Scale bars, 500 μm (Inset: 100 μm). e Growth of xenografted MIA-PaCa2 pancreatic tumors in male nude mice treated with vehicle control (n = 8 mice), cyst(e)inase (n = 8 mice), auranofin (n = 9 mice), or cyst(e)inase and auranofin in combination (n = 9 mice). f Isobologram of the effect of the combination of cyst(e)inase (Cys) and auranofin on BxPC3 and MIA-PaCa2 xenografts. All data represent mean ± s.e.m. *P < 0.05, **P < 0.01, ****P < 0.0001; compared to vehicle controls; repeated-measures two-way ANOVA (a, e) or two-way ANOVA (b, d) with Bonferroni’s method for multiple-comparison test

Back to article page