Fig. 3
From: Tumor inherent interferon regulators as biomarkers of long-term chemotherapeutic response in TNBC
Loss of IRF9 predicts rapid metastatic relapse. a IRF9 expression in TNBC primary tumors pre-chemotherapy, mid-chemotherapy, and post-chemotherapy was evaluated by IHC. Tissues were stained using rabbit anti-IRF9 antibody (5 μg/ml), IRF9 expression visualized using DAB prior to nuclear counterstain with hematoxylin. Representative images were taken of primary tumors with high and low staining, scale bars represent 100 μm. b Heat map representing IRF9 H score in the tumor cells of TNBC primary tumors throughout chemotherapy. Gray represents no evaluable sample available. c IRF9 H score in sequential TNBC primary tumors pre-chemotherapy and mid-chemotherapy. Error bars represent SEM. *p < 0.05 using Mann–Whitney U test. d Kaplan–Meier curve comparing distant relapse-free survival in patients who had IRF9-positive tumors or IRF9-negative tumors (positive IRF9 is determined as H score > 20) mid-chemotherapy. p values, hazard ratios, and confidence intervals calculated using a log-rank test (Mantel-Cox)
