Fig. 2
In vitro and in vivo efficacy of drug-loaded liposomes in RCC cell lines. 786-O (a) and A498 (b) cells were treated with various drug-loaded TTP-conjugated liposomes for 72 h. Then cell viability was determined with MTS assay. Dual-drug-loaded liposomes showed higher reduction in cell viability compared to single drug-loaded liposomes in all cell lines (n = 4 wells per dose). c 5 × 106 786-O cells were subcutaneously injected into the right flanks of 8-week-old male SCID mice. Tumors were allowed to grow until the average tumor size is ~400–500 mm3. Then mice were treated with drug-loaded liposomes (n = 1 mouse per treatment group) 3× per week for 3 weeks. Tumors were measured weekly and tumor volume was plotted to obtain the respective growth curves. In both cases dual-drug-loaded liposomes demonstrated stronger inhibition compared to single drug-loaded liposomes. Some of the mice were sacrificed before the completion of experiment due to ulceration of tumors. d Similar results were obtained in A498 xenografts (n = 1 mouse per treatment group).
