Fig. 1: GABP tetramer mediates the recruitment of activated ERK to mutant TERT promoter.
From: Synergistic activation of mutant TERT promoter by Sp1 and GABPA in BRAFV600E-driven human cancers
a Western blot was performed to determine the effect of GABPA knockdown on the levels of phosphorylated ERK (p-ERK) and total ERK (t-ERK) in the indicated cell lines. b ChIP-qPCR assay was performed to evaluate the effect of knocking down GABPA in the indicated cancer cells on the binding of p-ERK to TERT promoter. c Western blot was used to assess the effect of GABPB1/2 knockdown on the levels of p-ERK and t-ERK in the indicated cell lines. d ChIP-qPCR assay was performed to determine the effect of GABPA knockdown on the binding of p-ERK to TERT promoter in the indicated cells. e Co-IP with indicated antibodies followed by immunoblot (IB) showing the interaction between GABPA and p-ERK in A375 and 8305C cells knocking down GABPB and control cells. Data were shown as mean ± SD. *P < 0.05; **P < 0.01.
