Fig. 4: Mutation of Sp1 phosphorylation site attenuates GABPA binding and the activation of mutant TERT promoter.
From: Synergistic activation of mutant TERT promoter by Sp1 and GABPA in BRAFV600E-driven human cancers
The indicated cells were transfected with wild-type (Sp1-WT) or mutant Sp1 (Sp1-T739A) expression construct, and ChIP-qPCR assay was then performed to analyze the recruitment of GABPA (a), p-ERK (b), and HDAC1 (c) to mutant TERT promoter. d In vitro luciferase assay was performed to determine the effect of Sp1 phosphorylation on the activity of mutant TERT promoter in the indicated cells. e qRT-PCR assay was performed to evaluate the effect of Sp1 phosphorylation on TERT expression in the indicated cells. 18S rRNA was used as a reference gene. f ChIP-qPCR assay was performed to determine the effect of HDAC1 knockdown on the recruitment of GABPA at TERT promoter in A375 and 8305C cells expressing Sp1-T739A. Data were shown as mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001.
