Fig. 4: LINC00263 activates the NF-κB-signaling pathway through interaction with IKKα.
From: Annotation and cluster analysis of long noncoding RNA linked to male sex and estrogen in cancers
a RT-qPCR analysis of LINC00263 expression levels in different subcellular fractions in H1299 and H520 cells and LINC00263 mainly localized in cytoplasm. b The prediction of the interaction between LINC00263 and IKKα, IKKβ, IKKγ, P50, and P65. c RIP assays show the association between IKKα and LINC00263. d, e LINC00263 overexpression promoted P65 transfer into the nucleus. f–h The expression of related genes was measured with RT-qPCR in A549 e cells stably overexpressing LINC00263 and H1299. f Cells stably transfected with two distinct target shRNA vectors and control cells and tumors in nude mice h. i miRNA, LncRNA, RBP, TF interacting with LINC00263. j Mechanism of LINC00263 with miRNA and target genes. Data are shown as the mean ± SEM; n ≥ 3 independent experiments, two-tailed Student’s t-test: ns nonsignificant (P > 0.05), *P < 0.05, **P < 0.01, ***P < 0.001.
