Fig. 3: miRisk miRNA expression in low-risk and high-risk patients.
a–e Relative expression levels of the 5 miRisk miRNAs between low-risk and high-risk patients measured by small RNA sequencing. f–j Relative expression levels of the 5 miRisk miRNAs between low-risk and high-risk patients measured by qRT-PCR. The mean expression of triplicate measurements is shown. qRT-PCR expression was normalized according to the ΔCt method (CtmiRNA of interest − Ctmean of HK miRNAs). All statistical tests were two-tailed unpaired t tests. Error bars denote standard deviation. RPM reads per million. *p-value < 0.05, **p-value < 0.005, ***p-value < 0.0005, ****p-value < 0.00005, ns not significant.
