Fig. 5: Glial reactivity to glioma cells is patient-dependent, and astrocyte reactivity correlates with glioma CD71 expression.
From: A patient-designed tissue-engineered model of the infiltrative glioblastoma microenvironment
a Representative immunofluorescence images showing expression of reactivity markers GFAP (red, astrocytes) or CD68 (red, microglia) for glial cells alone (AM) or in tri-culture with G2, G34, and G528. Glioma cells (blue) are labeled only with DAPI, while CellTrackers label the astrocytes (green) and microglia (white). Some G528 cells also labeled with CD68. Scale bar is 100 µm. b Quantified number of GFAP+ astrocytes as a percent of total astrocytes. c Quantified number of CD68+ microglia as a percent of total microglia. d Correlation plot of GFAP and CD68 expression vs. GSC invasion data. e Correlation plot of GFAP and CD68 expression vs. GSC Ki67 expression data. f Correlation plot of GFAP and CD68 expression vs. GSC CD71 expression data. Spearman correlation coefficients and p values are shown in respective plots, with n = 3 trials per cell line.
