Fig. 1: ScRNA-seq profiling of TME in advanced BTC.

a The UMAP plot identified 4 main cell types in BTC, metastatic tissues and peripheral blood. b The number (right) and average proportion (left) of assigned cell types in different tissue types were presented. c UMAP plots of normalized marker expression of immune cells from all samples. d Bar plots depict percentage of B, T, NK, and myeloid cells in different tissues. Error bars indicate mean ± SEM. Two-tail paired t tests, **p < 0.01, *p ≤ 0.05.