Fig. 6: Representation of the TIL clusters at the Whole-Slide Image (WSI) level.
Two adenocarcinoma samples, labeled as low and high-risk by the trained model (MAD) from cohort D6. A It displays the H&E WSI for a sample labeled as low-risk. The overlaid grid describes patches of 2000 × 2000 pixel size which colors indicates tissue content and usefulness (green = High amount of tissue content, Yellow = Medium amount of tissue, and red = Low to no tissue content). Tiles allocated into the green grid were used for this representation. It depicts a zoomed-in region of interest at (B) 250 µm and (C) 50 µm. For each tile, TIL features and clusters were generated for the (D) for the low-risk sample. The colored-bar size indicates the log10 scale of the quantity of cells for the sample. The quantity of cells for each cluster is shown next to each colored dot and label identifier. A zoom-in patch is displayed alongside, highlighting the conformation of different cluster labels at (E) 250 µm and (F) 50 µm. Density estimation plot is further shown. The density estimation is performed through a probability density function in 2-D by a kernel density estimate, generating a binning grid area. The higher the quantity of data points (cells) that fall within the binning grid area, the higher the color intensity. This is performed for (G) individual clusters, (H) the ‘constructive’ clusters labeled as C (+) and ‘obstructive’ as negative C (−) and (I) density estimation of all the detected lymphocyte cells, highlighting the densest areas in red tonality (‘High’). Similarly, for high-risk samples, the samples were utilized from (J) H&E images, zoomed-in (K, L), (M) TIL clusters at WSI level and (N, O) zoom-in. Density plots for (P) clusters, (Q) ‘constructive’ and ‘obstructive,’ and (R) lymphocyte density plot.
