Fig. 2: Different levels of expression of FGFR2c revealed by RNA ISH showing FGFR2c expression in PDXs and PDXOs recapitulate corresponding patient tumours.
The micrograph images illustrate five EC patients and corresponding PDXs and PDXOs representing the different patterns of FGFR2c splice isoform expression. The first column represents the single model with very high FGFR2c splice isoform expression with an RNA ISH signal score of 4+ (cluster of signals without distinct dots or >10 clusters). The second column represents models with high FGFR2c expression (RNA ISH score of 3+ (>10 signals/cell with ā¤10 clusters). The third column represents a model of moderate FGFR2c expression with an RNA ISH score of 2+ (4-10 signals/cell without clustered dots) the fourth column represents a model with a low FGFR2c splice isoform RNA ISH score of 1 (2-3 signals/cell or >1 signals/10 tumour cells) and the fifth column is a model with negative FGFR2c splice isoform with RNA ISH score of 0 (<1 signal/10 tumour cells).
