Fig. 5: Significant cell death was observed in established PDXO models expressing FGFR2c following 72 hr treatment with 300 nM BGJ398. | npj Precision Oncology

Fig. 5: Significant cell death was observed in established PDXO models expressing FGFR2c following 72 hr treatment with 300 nM BGJ398.

From: Endometrial cancer PDX-derived organoids (PDXOs) and PDXs with FGFR2c isoform expression are sensitive to FGFR inhibition

Fig. 5: Significant cell death was observed in established PDXO models expressing FGFR2c following 72 hr treatment with 300 nM BGJ398.The alternative text for this image may have been generated using AI.

a Representative images from three different PDXO models expressing FGFR2c (top three panels) and two PDXO models without FGFR2c expression (bottom two panels) treated with vehicle (DMSO) or 300 nM BGJ398 for 72 h. Images were captured in XYZ stack at 10x microscopic objective view airy using an Inverted Laser rotatory Confocal Fluorescent Olympus Microscope with (FV1200 software). Green fluorescence shows esterase activity of live cells (Calcein AM), red fluorescence is generated upon binding of Ethidium homodimer-1 to DNA in damaged cells and nuclei were stained with DAPI. b Bar graph showing the proportion of live (green) and dead (red) cells in 5 independent PDO models treated with DMSO vehicle and 300 nM BGJ398. The experiment was performed in independent biological triplicate using independent PDXOs established from three different mice carrying each PDX as well as in technical triplicate. Live and Dead image analyses were performed using automated Fiji ImageJ2 software. **** two-sided student’s T-test P < 0.0001; Error bars indicate standard error of the mean (SEM), Scale bar 20 µm. DMSO Dimethyl-sulphoxide, DAPI Diamidino-2-phenylindole, FR2c + , Fibroblast Growth Factor Receptor 2c positive, FR2c- Fibroblast Growth Factor Receptor 2c negative, PDOs Patient-Derived Organoids, PDX Patient Derived Xenograft. NS not statistically significant.

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