Fig. 3: NCK enhances apoptosis and impedes cell-cycle progression in TNBC cells. | npj Precision Oncology

Fig. 3: NCK enhances apoptosis and impedes cell-cycle progression in TNBC cells.

From: Vertical pathway inhibition of receptor tyrosine kinases and BAD with synergistic efficacy in triple negative breast cancer

Fig. 3

a Hallmark analysis of the differential expressed genes (DEGs). Advanced bubble chart shows enrichment of hallmarks by DEGs commonly affected by NCK or NPB treatment. b Bar charts depicting DEGs commonly upregulated and downregulated by NCK or NPB treatment. c GSEA analyses of gene sets for cell cycle checkpoints. NES, normalized enrichment score. FDR, false discovery rate. Positive and negative NES indicate lower and higher expression in NCK when compared to NPB respectively. d Flow cytometry analysis of PI staining of cell cycle state of MDA-MB-231 and BT549 cells measured after treatment with 5 μM NCK or NPB using flow cytometry analysis as described in materials and methods. Data represent mean ± SD (n = 3). *P < 0.05, **P < 0.01, and ***P < 0.001. e Flow cytometry analysis of Annexin-V and propidium iodide (PI) staining of apoptotic cell death of MDA-MB-231 and BT549 cells measured after treatment with 5 μM NCK or NPB using flow cytometry analysis as described in materials and methods. Data represent means ± SD (n = 3). *P < 0.05, **P < 0.01, and ***P < 0.001.

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