Fig. 4: IHC and in-silico validation with independent datasets. | npj Precision Oncology

Fig. 4: IHC and in-silico validation with independent datasets.

From: Immunological subtyping of salivary gland cancer identifies histological origin-specific tumor immune microenvironment

Fig. 4

a PCA-based clustering ACC, MECA, and three independents, along with SDC and MEC, based on normalized expression. b Comparison of overall immune scores in the tumor microenvironment among histological subtypes using computational estimates. c Comparison of all TIME features illustrating differences in immunity among subtypes. d Immunohistochemistry (IHC) targeting infiltrating T-cells and immune cells labeled with CD3/CD45. e Comparison of significantly different cell type compositions by origin-derived subtypes. Comparison of (f) cancer stemness and (h) tumor suppressor activity. g Enriched GO terms in ID-derived subtypes using downregulated DEGs compared to ED-derived subtypes. Comparison of (i) tumor immune-related scores and molecules, specifically focusing on T-cell dysfunction, MDSC, and T-cell exclusion, as well as (j) immune signaling molecules, immunosuppressive factors, and subtypes of antigen-presenting cells.

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