Fig. 2: Patient-derived tumoroids recapitulate biological key features of original tumors.
From: Patient derived tumoroids of high grade neuroendocrine neoplasms for more personalized therapies
a Representative Hematoxylin and eosin (HE) staining and neuroendocrine diagnostic marker synaptophysin (SYN) immunolabeling in original tumor tissue and tissue-matched patient-derived (PD) tumoroids. Scale bar, 20 um. b Principal component analysis (PCA) plot of normalized gene expression in original tumor tissue and PD tumoroids. The color indicates patient identity with lines connecting matched original tumor and PD tumoroid samples. The variance explained by each principal component is indicated on the respective axis label. c Heatmap of gene expression for 8 neuroendocrine marker genes and the 8 top genes from the most strongly enriched gene ontology pathways. The pathways are “adaptive immune response” for the original tumor tissue and “post-translational protein modification” for the PD tumoroids. Gene expression values are centered and scaled row-wise (dark orange = highest expression, dark blue = lowest expression across all samples). Rows and columns are ordered by sample and gene identity.
