Fig. 4: Knockdown of UBE3C-LRP5 fusion suppresses clonogenic, migratory, invasive potential of head and neck cancer cells.
From: Recurrent UBE3C-LRP5 translocations in head and neck cancer with therapeutic implications
a Real-time PCR of LRP5, and UBE3C-LRP5 (v7) fusion variant in AW8507-UBE3C-LRP5 (v7) clones expressing scrambled siRNA or siRNAs targeting LRP5, or UBE3C-LRP5 (v7). b Real-time PCR of LRP5, and UBE3C-LRP5 (v1 and v2) fusion variants in NT-8e cells expressing scrambled siRNA or siRNAs targeting LRP5, UBE3C-LRP5 (v1), or UBE3C-LRP5 (v2). c, d, f, g Boyden chamber cell migration (c, f) and invasion assay (d, g) of AW8507-UBE3C-LRP5 (v7) (c, d) and NT-8e cells (f, g) expressing scrambled siRNA or siRNA targeting LRP5 or the UBE3C-LRP5 fusion variants. The bar plots depict the number of cells migrated or invaded in scrambled siRNA control or siRNAs targeting LRP5, or UBE3C-LRP5 fusion variants. e, h Clonogenic cell survival assay of AW8507-UBE3C-LRP5 (v7) (e) and NT-8e cells (h) expressing scrambled siRNA or siRNA targeting the UBE3C-LRP5 fusion variants or LRP5. The bar plots on the right indicate the number of colonies formed in each group. Data were shown as means ± SD. p values are from the Student’s unpaired t-test and are denoted as ns (not significant); *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. Scale bar = 400 µM.
