Table 2 Different conditions for the pBT tissues with corresponding cell lines combining DNAm and CNV

From: Evaluating cell culture reliability in pediatric brain tumor primary cells through DNA methylation profiling

Condition

DNAm

CNV profile

Observed cases (%)

Representative cases

Tissue (T)

Cell line*

1

Maintained

Coincident

25

HGG_T8

HGG_CSFL8c_3D

2

Maintained

Similar

6

LGG_T11

LGG_CSE11_2D

3

Maintained

Different

5

DMG_T1

DMG_CSFE1_2D

4

Unfaithful

Coincident

9

HGG_T1

HGG_CSFE1b_3D

5

Unfaithful

Similar

33

MB_SHHB_T3

MB_CSFL3_3D

6

Unfaithful

Different

22

HGG_T4

HGG_CSFE4a_2D

  1. Tumor tissues (T) and cell lines (C) are named with the tumor acronym and progressive numbers, *Cell lines were named according to the nomenclature below: primary cultures (C), either as serum-free (SF) or serum-supplemented medium (S) and considering early (E) and long (L) passages in culture, in both two-dimensional (2D) and three-dimensional (3D) conditions. Different cell lines method cultures are indicated with italic letters.
  2. DNAm DNA methylation, CNV copy number variations, LGG low-grade gliomas, HGG high-grade gliomas, DMG diffuse (pontine) midline gliomas H3 K27M-altered, MB medulloblastoma.
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