Fig. 2: Functional PD-1 ligand binding associates with response to anti-PD-1 therapy, independent of treatment line.

a. Cohort distribution into responders (R) and non-responders (NR) based on treatment lines. b. Survival curves indicating non-significant differences in progression-free survival time between responders and non-responders (log-rank p = 0.13, n = 29) based on the line of treatment. c. Survival curves indicating non-significant differences in overall survival time between responders and non-responders (log-rank p = 0.13, n = 29) based on line of treatment. d. Survival curves showing significant differences in progression-free survival time between responders and non-responders (log-rank p = 0.0018, n = 29) based on PD1 score. e-g. Boxplots displaying PD-1, PD-L1, and PD-L2 scores for responders (R) and non-responders (NR), segregated by line of treatment. h Dot-plot depicting the relationship between PD-L1 and PD-L2 scores. The line of treatment is indicated by the shape of the dots: triangles for 1st line and circles for 2nd and 3rd lines. i Swimmer plots showing the time from treatment initiation layered by treatment lines and PD1 score; teal = responder, red = non-responder, triangle = beginning of response, circle= end of response, line = duration of response, arrow = ongoing response.