Fig. 5: Inhibition of PIM proteins leads to changes in gene expression patterns.

a Volcano plot showing the results for multiple GSEA-signatures between M07e-JAK3^Q988P cells untreated (referred as untreated) and M07e-JAK3^Q988P cells treated with PIM447 for 24 hours (referred as PIM447). The selected GSEA-signatures correspond to different cellular processes that have been implicated in cancer. Those signatures with a confidence rate above 95% are highlighted in red. Data derived from the analysis of the significantly enriched signatures are shown in the adjacent chart: NES, normalized enrichment score; P, nominal p value; FDR, false discovery rate. b GSEA-plots for adipogenesis, oxidative phosphorylation, steroid biosynthesis and rRNA processing in M07e-JAK3^Q988P cells untreated and treated with PIM447. c Western blot for p-S6, S6, p-4E-BP1 and 4E-BP1 in M07e cells transduced with JAK3^Q988P, JAK1^V658F or STAT5^N642H and treated with rapamycin (10 nM, 50 nM or 100 nM) or left untreated. d Cell growth (left) and viability (right) assays of M07e cells transduced with JAK3^Q988P, JAK1^V658F or STAT5^N642H, treated with rapamycin (10 nM, 50 nM or 100 nM) and referred to untreated cells. Statistical comparisons are made against untreated cells. The graphics show the mean ± standard deviation (s.d.) after three independent experiments. All images are representative examples of at least three independent experiments.