Fig. 3: Tumor cell characterization.

Untreated or IFNγ-treated (200 IU/ml for 48 h) tumor cells were analyzed via flow cytometry. Percentage of HLA class I- and II-positive cells were measured (a). PD-L1-positive tumor cells were detected and percentages (b) and median fluorescence intensity (c) are depicted. Percentages of CD80/86- (d) and PI-9-positive cells (e) were determined. Depicted are means of 3–5 biological replicates and the respective standard deviation.