Fig. 6: VIM was a downstream interacting protein target of XRCC2.

a IP experiment capturing XRCC2 interacting proteins for subsequent silver nitrate staining. b Scoring and sorting of the main interacting proteins of XRCC2, including the secondary mass spectrum of characteristic peptides of the interacting protein Vimentin. c Verification of XRCC2 and vimentin interaction through co-IP assays. d, e Protein level detection of VIM after XRCC2 knockdown or overexpression, as assessed by Western blot experiments. f Following XRCC2 overexpression in PC9 cells, treatment with cycloheximide (CHX) (100 ng/mL) over various time points (0, 1 h, 2 h, 4 h, 8 h, and 12 h) with Western blot used to detect Vimentin expression. NC Negative Control, OE Overexpression XRCC2. g XRCC2 knockdown in A549 cells treated with MG132 (10 μM) for 6 h, with Western blot revealing the impact of XRCC2 knockdown on vimentin expression. h Western blot analysis of the ubiquitination level of Vimentin protein after XRCC2 knockdown in A549 cells. i Co-localization observation of XRCC2 and Vimentin in cells using laser confocal microscopy.