Fig. 1: Setup and validation of a microarrayed platform for immuno-oncology studies.

a Illustration of the differences between standard (top) and microarrayed (bottom) culture systems for tumoroids. b Representative brightfield images of standard (left) and microarrayed (right) tumoroid cultures. Scale bar, 500 µm. c Scheme of the experimental workflow followed in this study. d Representative images of microarrayed co-cultures of tumoroids and T cells at the indicated ratios (left) and time (top). Scale bar, 250 µm. e Tumoroid area over time at the indicated cell ratios and time. **P < 0.01, ***P < 0.001 (Kruskal–Wallis and Dunn’s tests, n = 38 (top), and ANOVA and Tukey’s tests, n = 37 (bottom)). f Measurement of the PI signal at day 2 in the indicated cell ratio conditions. **P < 0.01, ***P < 0.001 (Kruskal–Wallis and Dunn’s tests, n = 32). In e, f, data represent mean ± SEM.