Fig. 6: The CCL2 expression in SFRP2_CAFs may be regulated by NFIX.

A SCENIC transcription factors correlation and clustering results showed 12 distinct modules labeled as M1-12 in GSE181919. B The UMAP embedding plot for M1 module in GSE181919. C Integrating the JASPAR database with the SCENIC results revealed 36 common transcription factors in GSE181919. D The UMAP embedding plot for SFRP2_CAFs annotation and NFIX in GSE181919. E SCENIC transcription factors correlation and clustering results showed 14 distinct modules labeled as M1-14 in GSE188737. F The UMAP embedding plot for M1 module in GSE188737. G Integrating the JASPAR database with the SCENIC results revealed 37 common transcription factors in GSE188737. H The UMAP embedding plot for SFRP2_CAFs annotation and NFIX in GSE188737. I Dot plot of CCL2 features according to the GSE181919 cell types, the scaled mean expression value within each cell type, visualized by dot color; the dot size represents the fraction of cells in the cluster with corresponding values. J Dot plot of CCL2 features according to the GSE188737 cell types, the scaled mean expression value within each cell type, visualized by dot color; the dot size represents the fraction of cells in the cluster with corresponding values. K The correlation (R = 0.304, p = 3.38e−12) between CCL2 and NFIX in TCGA-HNSC cohort. L The bar graph demonstrates that the transfection efficiency of siNFIX is excellent in CAFs. M, N The qPCR and western blot results indicate alterations in the expression levels of CCL2 following the siNFIX perturbation. P value = (* <0.05; ** <0.01; *** <0.001, **** <0.0001).