Fig. 2: NSUN2 promotes cancer cells proliferation and migration in PRAD.

A, B Representative immunoblotting assays and qPCR analyses demonstrate the efficiency of transfection in C4-2 (A) and 22Rv1 (B) cell lines. C, D Quantification results from the CCK-8 proliferation assay showing the effects of NSUN2 knockdown (C) and overexpression (D) in C4-2 cells. E, F Quantification of the CCK-8 proliferation assay for 22Rv1 cells, illustrating the effects of NSUN2 knockdown (E) and overexpression (F). G, H Representative images and quantification of invasion assays in NSUN2-knockdown, NSUN2-overexpressing, and control C4-2 (G) or 22Rv1 (H) cells. Scale bar = 400 μm. Quantification data is also provided. I, J Representative images of the colony formation assay in C4-2 (I) and 22Rv1 (J) cells, with corresponding quantification data. K Representative images and quantification of migration assays for NSUN2-knockdown, NSUN2-overexpressing, and control 22Rv1 cells. Scale bar: 400 μm. Quantification data is also provided. L Tumor images from nude mice implanted with parental, NSUN2 knockdown, or overexpressing 22Rv1 cells (n = 5). M, N Tumor mass (M) and growth curves (N) for implanted tumors formed by parental, NSUN2 knockdown, or overexpressing 22Rv1 cells. O Representative HE staining and IHC images showing NSUN2, TRIM28, and Ki67 staining in implanted tumors from NSUN2 knockdown or overexpressing cells. 20×: Scale bar = 100 μm, 40×: Scale bar = 50 μm.