Fig. 6: TRIM28 promotes cancer cells proliferation and migration in vitro. | npj Precision Oncology

Fig. 6: TRIM28 promotes cancer cells proliferation and migration in vitro.

From: FOXA1-dependent NSUN2 facilitates the advancement of prostate cancer by preserving TRIM28 mRNA stability in a m5C-dependent manner

Fig. 6

A KEGG and GSEA analyses indicate pathways enriched in samples with high TRIM28 expression. B Representative immunoblotting assay and quantitative PCR (qPCR) demonstrate the efficiency of TRIM28 knockdown and overexpression in C4-2 cells. Quantification of CCK-8 proliferation assays showing the effects of TRIM28 knockdown (C) and overexpression (D) in C4-2. E Representative images and quantification from the colony formation assay comparing TRIM28 knockdown and overexpression in C4-2. F Representative images and quantification of invasion assays for TRIM28-knockdown, TRIM28-overexpression, and control C4-2 cells. Scale bar: 400 μm. G Representative immunoblotting assay confirming the efficiency of TRIM28 knockdown and overexpression in 22Rv1 cells. Quantification of CCK-8 proliferation assays in TRIM28 knockdown (H) and overexpression (I) in 22Rv1 cells, presented as mean ± SD (n = 6). J Images of colony formation assay in TRIM28 knockdown and overexpression 22Rv1, with corresponding quantification data. K Transwell assay results comparing TRIM28 knockdown and overexpression in 22Rv1. Scale bar: 400 μm. L Representative images of migrated TRIM28-knockdown, TRIM28-overexpression, and control 22Rv1 cells, with quantification data included. Scale bar: 400 μm.

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