Fig. 2: GPC2 CAR T cells are also functional against adult glioblastoma.
From: Defining the extracellular matrix for targeted immunotherapy in adult and pediatric brain cancer
A Cell surface proteomics of 8 primary brain cancers (including low and high-grade gliomas) showing the presence of Extracellular matrix components detected at the cell surface (gray indicates lack of detection). B Top 14 Extracellular Matrix components based on adult brain cancer cell surface proteomics for potential use as immunotherapy targets as predicted by an ImmunoTar score, with higher scores indicating stronger immune targeting potential. C Quantification of PI uptake over the 72 h of an IncuCyte killing assay of 2:1 ratio of previously described CD8 + GPC2-CAR T cells, CD19 negative control CAR T cells and control T cells co-cultured with pediatric glioblastoma U251 tumor cells over 72 h. Histology was performed on 40 primary Adult High Grade Glioma tumor samples [across a 30-patient cohort] to determine extend of endogenous T cells infiltration (dual anti-CD4+ and anti-CD8+ stains) and ECM deposition using Alcian Blue (stains glycosaminoglycans) and Massons Trichome (stains collagen) stains. Staining was performed on serial cut tumor sections and scored by an anatomical pathologist. D Here we show a bubble graph comparing T Cell infiltration (H-score from anti-CD4+ and anti-CD8+ stain) and ECM deposition (composite score of the Alcian blue H-score multiplied by the Massons Trichome H-Score) in adult brain cancers, the correlation between these two factors yielded a Pearson’s correlation coefficient of 0.184.
