Fig. 4: Collagen and other extracellular matrix glycoproteins are diffuse throughout adult and pediatric brain cancers.
From: Defining the extracellular matrix for targeted immunotherapy in adult and pediatric brain cancer
Like previously described histology was performed on primary adult and pediatric brain cancers, here we show Massons Trichome (stains collagen) staining and the corresponding H&E sections from (A, B) two different adult glioblastomas (aGBM) (score 1 and score 3, respectively) and two pediatric high-grade gliomas including (C) a pediatric glioblastoma (pGBM) (score 2) and an (D) Diffuse Intrinsic Pontine Glioma (DIPG) (score 2) (scale bar = 100 μm); The scoring of Massons Trichome, and presence of collagen, staining for adult and pediatric cohorts, by diagnosis, is quantified in (E) and (F), respectively; Cell surface proteomics of (G) 8 primary adult brain cancers (including low and high-grade gliomas) and (H) 2 primary pediatric diffuse intrinsic pontine gliomas (DIPGs) [5 replicates for each tumor] showing the presence of collagens and other extracellular matrix glycoproteins detected at the cell surface (gray indicates lack of detection); Comparison of the presence (mean IBAQ scores) of collagens and glycoproteins identified by cell surface proteomics in both primary human brain cancers and model cell lines used in preclinical models with specific comparisons between (I) primary adult brain cancers (low and high grade gliomas) and GBM cell lines (U87, SW1088, U118, H4, A1172 and U251) and (J) primary pediatric DIPG tumors and patient-derived DIPG model cell lines.
