Fig. 3: Expression profiles revealed distinct clusters of epithelial cells in CXPA and PG.

A Heatmap illustrating gene expression signatures parsed from a representative tumor (CXPA-2) using NMF. Each expression signature is numbered, with corresponding genes displayed (right). CXPA-2 refers to T2. B Pearson’s correlation clustering of 30 expression signatures. Four regions comprising clusters of highly correlated signatures were identified as meta-signatures. C Heatmap depicting the expression levels of the top 50 marker genes in each meta-signature. D UMAP plots displaying the distribution of four meta-signatures after using the “AddModuleScore” function. E UMAP plots demonstrating the distribution of four cell states and sample origins in CXPA and PG epithelial cells. F GO and KEGG enrichment analyses of marker genes for each cell state. G Heatmap depicting differences in TF activity across epithelial clusters of CXPA and PG (scored by SCENIC).