Fig. 1: ARHGAP4 is highly expressed in CRC and is associated with poor prognosis.

A Gene difference analysis heat map of cancer and adjacent tissues of COAD and READ in TCGA database; B Venne diagram of the intersection of the gene set after the difference analysis of CRC data in TCGA and the RhoGAPs family gene members; C–E OS, DSS and PFI analysis were performed on patients with high and low expression of ARHGAP4 according to the median expression level by TCGA data; F RFS survival analysis of ARHGAP4 high and low expression groups was performed through the GEPIA database (including TCGA and GTEX data); G Lasso dimensionality reduction analysis of eight highly expressed genes in the intersection genes through TCGA data, and finally only ARHGAP4 was eligible; H KM overall survival curve analysis was performed on 78 patients with primary CRC from the Provincial Hospital Affiliated to Fuzhou University, and the patients were divided into high and low expression groups by median; I and J ARHGAP4 paired difference analysis in GSE44076 and TCGA; K Differential expression analysis of ARHGAP4 in pan-cancer; L The expression difference of ARGHGAP4 in CRC was verified by IHC staining of cancer and adjacent tissues in postoperative resection specimens of primary CRC patients in Fuzhou Provincial Hospital Affiliated to Fuzhou University; M–O The correlation between ARHGAP4 expression and T stage, N stage and clinical stage was analyzed in 78 patients with primary CRC from the Provincial Hospital Affiliated to Fuzhou University.