Fig. 6: In vivo treatment of NB PDX models with a combination of Auranofin and COJEC.

A Schematic summary of the experimental design for the Auranofin in vivo treatment. B Normalized tumor growth curves and survival Kaplan Meier curves for experiment described in (A). Treatment groups: Control (n = 7, orange), Auranofin (n = 7, green), COJEC (n = 8, blue), Combination (n = 8, pink). Log rank statistical analysis for survival, and mixed-effects analysis multiple comparison for tumor growth (Control-Auranofin $ P-value < 0.05; Control-Combination, *P-value < 0.05, and ** P value < 0.01; COJEC-Combination # P-value < 0.05). C GSEA analysis of RNA expression signatures from tumors from (B). Comparison of treatment groups against the control group using the Hallmarks signature database. D CD71 (green) and 4HNE (red) fluorescence immunostaining images and quantitation, two-way ANOVA analysis with multiple comparison with Tukey correction (4HNE positive cells comparison: *P-value < 0.05, **P-value < 0.01, ***P-value < 0.001; Negative cells comparison: ^##P-value < 0.01, ^###P-value < 0.001). Oxidative stress marker 8-hydroxy-2’-deoxyguanosine (8OhdG) DAB immunostaining images and quantitation, non-parametric Kruskal-Wallis test with multiple comparison with Dunn’s test correction (*P-value < 0.05, **P-value < 0.01, ***P-value < 0.001). NCOA4 DAB immunostaining images and quantitation, non-parametric Kruskal-Wallis test with multiple comparison with Dunn’s test correction (*P-value < 0.05, **P-value < 0.01, ***P value < 0.001). Prussian Blue iron staining images and quantitation, one-way ANOVA analysis with multiple correction comparison with Dunnett correction (*P-value < 0.05). TH (green) and SOX9 (magenta) fluorescence immunostaining images and quantitation, two-way ANOVA analysis with multiple comparison with Tukey correction (SOX9 positive cells comparison: ***P-value < 0.001; TH positive cells comparison: ^###P-value < 0.001). All scale bars represent 100 µm.