Fig. 5: Prkci interacted with and induced phosphorylation of c-Myc in colorectal cancer cells.
From: Prkci acts a pro-proliferation factor in colorectal cancer
A, B Co-immunoprecipitation (Co-IP) of Prkci with c-Myc, confirming their interaction. C Western blot analysis of the phosphorylation level of c-Myc in Prkci knockout cells. D Schematic of c-Myc domains. E Co-IP of Prkci with c-Myc 1–150 aa segments, identifying the main interaction region. F Co-IP of Prkci with c-Myc 1–50 aa segments, identifying the main interaction region. G Western blot analysis of Prkci-mediated phosphorylation of c-Myc at serine 21. H In vitro kinase assay using Flag-Prkci protein, His-tagged c-Myc WT and S21A mutant proteins, followed by western blot. I Western blot showing effects of phosphorylation-inactive (S21A) and phosphomimetic (S21E) mutations of c-Myc on the polyubiquitination levels of c-Myc. Each IB assay was performed in triplicate, yielding consistent results.
