Fig. 2: Multiplex staining evaluating tumor and stroma immune infiltration in samples from the MITO16A case material. | npj Precision Oncology

Fig. 2: Multiplex staining evaluating tumor and stroma immune infiltration in samples from the MITO16A case material.

From: Tumour-infiltrating leucocytes as prognostic biomarkers of bevacizumab-treated ovarian cancer patients results from the phase IV MITO16A/MaNGO OV-2 clinical trial

Fig. 2: Multiplex staining evaluating tumor and stroma immune infiltration in samples from the MITO16A case material.

A Scheme of the multiplex analyses using the OPAL Multiplex Assay coupled with multispectral image acquisition. Centralized tumor samples were processed, and single FFPE tissue slides were stained with the indicated antibodies for MIF. Stained sections were acquired with a Nikon microscope coupled with a multispectral camera, and acquired images were analyzed with the InForm software to define the number of immune cells infiltrating the tumors (PanCK-positive areas) and the surrounding stroma. Created with BioRender.com. B On the left, a typical image of immune cells infiltrating the tumor (color code as in A). Scale bar is 100 μm. On the right, pie charts report the number of analyzed slides that proved adequate or not for subsequent analyses after the multi-staining process. C Correlation between tumor and stroma infiltration in the same sample from CD8+ (left graph) and CD68+ (right graph) immune cells. D Graphs reporting the percentage of CD8 and CD68 immune-excluded (Neg) and immune-infiltrated (Pos) samples, as indicated.

Back to article page