Fig. 2: ZNF217 depletion impairs ovarian cancer cell proliferation and metastatic potential in vitro. | npj Precision Oncology

Fig. 2: ZNF217 depletion impairs ovarian cancer cell proliferation and metastatic potential in vitro.

From: ZNF217 promotes ovarian cancer progression by impacting multiple pivotal steps in the metastatic process

Fig. 2

A Western blot showing stable shRNA mediated ZNF217 knockdown in ovarian cancer cells. B ZNF217 knockdown in OVCA420 and TYK-Nu cells causes a reduction in cell proliferation (n = 3). C Transwell migration assay show that ZNF217 depletion reduces ovarian cancer cell migration (n = 3). D ZNF217 knockdown decreases the ability of ovarian cancer cells to invade through Matrigel (n = 3). E ZNF217 knockdown decreases attachment of OVCA420 cells to fibronectin coated plates (n = 3). F ZNF217 knockdown decreases attachment of OVCA420 cells to collagen coated surface (n = 3). G Western blot confirming successful re-expression of shRNA resistant ZNF217 in OVCA420 and TYK-Nu ZNF217 knockdown cells. H Restoration of ZNF217 expression using an shRNA-resistant form of ZNF217 rescues defects in cell proliferation in OVCA420 and TYK-Nu cells (n = 3). I Restoration of ZNF217 expression using an shRNA-resistant form of ZNF217 rescues defects in cell migration in OVCA420 and TYK-Nu cells (n = 3). J Restoration of ZNF217 expression using an shRNA-resistant form of ZNF217 rescues defects in OVCA420 and TYK-Nu invasion through the Matrigel (n = 3).

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