Fig. 6: ZNF217 overexpression causes oncogenic transformation in immortalized fallopian tube secretory epithelial cells.

A Western blot confirming stable ZNF217-V5 expression in FT237 and FT282 cells. B ZNF217 overexpression in FT237 and FT282 cells increases proliferation (n = 3). C Transwell migration assay shows that ZNF217 overexpression in FT237 and FT282 cells increases their ability to migrate (n = 3). D ZNF217 overexpression in FT237 and FT282 cells enhances their ability to invade through Matrigel (n = 3). E Representative bright field image and quantification showing that ZNF217 overexpression in FT237 (n = 7) and FT282 (n = 5) cells increases both the formation and size of spheroids when grown under low attachment conditions for 24 hours. F Western blot showing ZNF217 overexpression induces the expression of EMT associated genes in FT237 and FT282 cells.